Terri attempts to shoot the anaconda to save him, but Serone knocks her gun away and the snake devours Gary. Serone attempts to capture the snake, but it coils around Gary, crushing him. That night, the anaconda attacks the boat crew. He promises that if the crew helps him find the snake, he will help them get out alive. The others try to find Mateo while Serone kills a monkey to use as anaconda bait. A photograph in an old newspaper reveals that Mateo, Serone, and the poacher were working together to hunt animals, including snakes. Mateo gets lost and is the first to be killed by the anaconda near the wreckage of the poacher's boat. Serone performs an emergency cricothyrotomy, seemingly saving Cale's life, but soon after, he takes over the boat, forcing the crew to help him achieve his true goal: hunting down a giant record-breaking green anaconda he had been tracking, which he believes he can capture alive. Eventually, Cale is stung by a wasp, and an allergic reaction swells up his throat and leaves him unconscious. Most of the crew are uncomfortable around Serone, and Cale clashes with him several times about Shirishama lore. The group encounters stranded Paraguayan snake hunter Paul Serone, who convinces them he can help them find the Shirishamas. The crew includes director Terri Flores, cameraman and childhood friend Danny Rich, production manager Denise Kalberg, Denise's boyfriend and sound engineer Gary Dixon, narrator Warren Westridge, anthropologist Professor Steven Cale, and boat skipper Mateo. Meanwhile, a film crew is shooting a documentary about the Shirishamas, a long-lost indigenous Amazonian tribe. While it breaks through the boat and attempts to catch the poacher, he commits suicide by shooting himself to prevent it from killing him. On the Amazon River, a poacher hides from an unknown creature in his boat.